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Px Wire is AVAC's quarterly update covering the latest in the field of biomedical HIV prevention research, implementation and advocacy. This issue of Px Wire features an advocates’ guide to the “sieve analysis” from the RV144 vaccine trial. This latest follow-up investigation provided further detail about the partial protection associated with the vaccine strategy. This issue also updates readers with the latest on trials of ARV-containing vaginal rings—the next frontier in microbicide development.