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Px Wire is AVAC's quarterly update covering the latest in the field of biomedical HIV prevention research, implementation and advocacy. This issue reviews highlights from the biannual International Microbicides Conference (M2010) held in May, including discussions of the potential opportunities and challenges of ARV-based prevention in topical and oral forms, new developments in delivery systems for microbicides and increasing recognition of rectal microbicides as a key area for exploration. This issue also covers what to expect with the forthcoming results of CAPRISA 004.